This article systematically delineates the core principles and technical advantages of ONT direct RNA sequencing. The article discusses how current changes induced by RNA molecules are captured by nanopores in real time. It explains how this process can directly analyze the complete transcript structure and identify modification sites, avoiding the amplification bias of traditional me... https://www.cd-genomics.com/epigenetics/resource-ont-direct-rna-sequencing-principles-workflow-analysis.html